Spider DNA degrade quickly? / DNA labah-labah rosak cepat?

Situation:
The spider collected from the field were stored in 75% ethanol. The specimens were left in the chalet for about a month in the forest. As soon as we got back to our lab, the spider specimen were stored in temperature -20oC freezer. After 18 months, we tried to extract the DNA and amplify at the COI (600-700 bp) and 18S (900-1100 bp) region, we found out that most DNA had been degraded. This is not surprising as previous report also mentioned that spider DNA degrade very quickly in less than 2 months if stored in 75% ethanol.

Recommendation: PLEASE extract the DNA as soon as possible.


Situasi:
Labah-labah yang dikumpul dari lapangan telah disimpan dalam 75% etanol. Spesimen dibiarkan di chalet selama kira-kira sebulan di dalam hutan. Sebaik sahaja kami kembali ke makmal, spesimen labah-labah telah disimpan di dalam peti sejuk pada suhu-20oC. Selepas 18 bulan, kami cuba untuk mengekstrak DNA dan menguatkan menggunakan COI (600-700 bp) dan 18S (900-1100 bp), namun kita mendapati bahawa kebanyakan DNA telah rosak. Ini tidak menghairankan kerana laporan sebelum ini juga menyebut bahawa labah-labah DNA rosak dengan cepat dalam masa kurang dari 2-6 bulan jika disimpan dalam 75% etanol.

Nasihat: SILA mengekstrak DNA spesimen secepat mungkin.